Identification and expression of a murine cytomegalovirus early gene coding for an Fc receptor

Episode von Medizin - Open Access LMU - Teil 11/22 von Ludwig-Maximilians-Universität München · 1. Januar 1994

Several herpesviruses, including cytomegalovirus, induce receptors for the Fc domain of murine immunoglobulin G (IgG) molecules. Viral genes coding for these receptors have been characterized only for alphaherpesviruses. In this report, we describe a new approach that led to the identification of an Fc receptor (FcR) of murine cytomegalovirus (MCMV). The Fc fragment of IgG precipitated glycoproteins (gp) of 86 to 88 and 105 kDa from MCMV-infected cells. Deglycosylation by endoglycosidase F resulted in a protein with a molecular mass of 64 kDa. Injection of complete MCMV DNA or of DNA fragments, and the subsequent testing of cytoplasmic binding of IgG by immunofluorescence microscopy, was used to search for the coding region in the MCMV genome. The gene was located in the HindIII J fragment, map units 0.838 to 0.846, where an open reading frame of 1,707 nucleotides predicts a gp of 569 amino acids with a calculated molecular mass of 65 kDa. The sequence of this gp is related to those of the gE proteins of herpes simplex virus type 1 and varicella-zoster virus. The defined length of the mRNA, 1,838 nucleotides, was in agreement with that of a 1.9-kb RNA expressed throughout the replication cycle, starting at the early stages of infection. Expression of the gene fcr1 by recombinant vaccinia virus resulted in the synthesis of gp86/88 and gp105, each with FcR properties, and the correct identification of the gene encoding the FcR was confirmed by the DNA injection method.

Weitere Episoden von „Medizin - Open Access LMU - Teil 11/22“

• Antibodies Are Not Essential for the Resolution of Primary Cytomegalovirus Infection but Limit Dissemination of Recurrent Virus 1. Januar 1994
• Bilaterale massive makronoduläre Nebennierenhyperplasie 1. Januar 1994
• CGM2, a Member of the Carcinoembryonic Antigen Gene Family is Down- Regulated in Colorectal Carcinomas 1. Januar 1994
• Chronic Progressive External Ophthalmoplegia Is Associated with a Novel Mutation in the Mitochondrial tRNA(Asn) Gene 1. Januar 1994
• Clonal Composition of Human Adrenocortical Neoplasms 1. Januar 1994
• Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans 1. Januar 1994
• Cutaneos larva migrans in travelers 1. Januar 1994
• Differential expression of synaptophysin and synaptoporin during pre- and postnatal development of the hippocampal network 1. Januar 1994
• Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens 1. Januar 1994
• Exploring the functional domain and the target of the tetanus toxin light chain in neurohypophysial terminals 1. Januar 1994
• Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization 1. Januar 1994
• Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus 1. Januar 1994
• Fibronectin in human gallbladder bile: cholesterol pronucleating and/or mucin "link" protein? 1. Januar 1994
• Folgen importierter Tropenkrankheiten in Deutschland 1. Januar 1994
• Gene Structure, cDNA Sequence, and mRNA Distribution 1. Januar 1994

Alle Episoden von Medizin - Open Access LMU - Teil 11/22 · Mehr Bildung-Podcasts