Exploring the functional domain and the target of the tetanus toxin light chain in neurohypophysial terminals

Episode von Medizin - Open Access LMU - Teil 11/22 von Ludwig-Maximilians-Universität München · 1. Januar 1994

The tetanus toxin light chain blocks calcium induced vasopressin release from neurohypophysial nerve terminals. Here we show that histidine residue 233 within the putative zinc binding motif of the tetanus toxin light chain is essential for the inhibition of exocytosis, in the rat. The zinc chelating agent dipicolinic acid as well as captopril, an inhibitor of zinc-dependent peptidases, counteract the effect of the neurotoxin. Synthetic peptides, the sequences of which correspond to motifs present in the cytoplasmic domain of the synaptic vesicle membrane protein synaptobrevin 1 and 2, prevent the effect of the tetanus toxin light chain.

Our results indicate that zinc bound to the zinc binding motif constitutes the active site of the tetanus toxin light chain. Moreover they suggest that cleavage of synaptobrevin by the neurotoxin causes the inhibition of exocytotic release of vasopressin from secretory granules.

Weitere Episoden von „Medizin - Open Access LMU - Teil 11/22“

• Antibodies Are Not Essential for the Resolution of Primary Cytomegalovirus Infection but Limit Dissemination of Recurrent Virus 1. Januar 1994
• Bilaterale massive makronoduläre Nebennierenhyperplasie 1. Januar 1994
• CGM2, a Member of the Carcinoembryonic Antigen Gene Family is Down- Regulated in Colorectal Carcinomas 1. Januar 1994
• Chronic Progressive External Ophthalmoplegia Is Associated with a Novel Mutation in the Mitochondrial tRNA(Asn) Gene 1. Januar 1994
• Clonal Composition of Human Adrenocortical Neoplasms 1. Januar 1994
• Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans 1. Januar 1994
• Cutaneos larva migrans in travelers 1. Januar 1994
• Differential expression of synaptophysin and synaptoporin during pre- and postnatal development of the hippocampal network 1. Januar 1994
• Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens 1. Januar 1994
• Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization 1. Januar 1994
• Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus 1. Januar 1994
• Fibronectin in human gallbladder bile: cholesterol pronucleating and/or mucin "link" protein? 1. Januar 1994
• Folgen importierter Tropenkrankheiten in Deutschland 1. Januar 1994
• Gene Structure, cDNA Sequence, and mRNA Distribution 1. Januar 1994
• Gnathostomiasis nach Aufenthalt in Thailand. 1. Januar 1994

Alle Episoden von Medizin - Open Access LMU - Teil 11/22 · Mehr Bildung-Podcasts