Differential expression of synaptophysin and synaptoporin during pre- and postnatal development of the hippocampal network

Episode von Medizin - Open Access LMU - Teil 11/22 von Ludwig-Maximilians-Universität München · 1. Januar 1994

The closely related synaptic vesicle membrane proteins synaptophysin and synaptoporin are abundant in the hippocampal formation of the adult rat. But the prenatal hippocampal formation contains only synaptophysin, which is first detected at embryonic day 17 (E17) in perikarya and axons of the pyramidal neurons. At E21 synaptophysin immunoreactivity extends into the apical dendrites of these cells and in newly formed terminals contacting these dendrites. The transient presence of synaptophysin in axons and dendrites suggests a functional involvement of synaptophysin in fibre outgrowth of developing pyramidal neurons. Synaptoporin expression parallels the formation of dentate granule cell synaptic contacts with pyramidal neurons: the amount of hippocampal synaptoporin, determined in immunoblots and by synaptoporin immunostaining of developing mossy fibre terminals, increases during the first postnatal week. Moreover, in the adult, synaptoporin is found exclusively in the mossy fibre terminals present in the hilar region of the dentate gyrus and the regio inferior of the cornu ammonis. In contrast, synaptophysin is present in all synaptic fields of the hippocampal formation, including the mossy fibre terminals, where it colocalizes with synaptoporin in the same boutons. Our data indicate that granule neuron terminals differ from all other terminals of the hippocampal formation by the presence of both synaptoporin and synaptophysin. This difference, observed in the earliest synaptic contacts in the postnatal hippocampus and persisting into adult life, suggests distinct functions of synaptoporin in these nerve terminals.

Weitere Episoden von „Medizin - Open Access LMU - Teil 11/22“

• Antibodies Are Not Essential for the Resolution of Primary Cytomegalovirus Infection but Limit Dissemination of Recurrent Virus 1. Januar 1994
• Bilaterale massive makronoduläre Nebennierenhyperplasie 1. Januar 1994
• CGM2, a Member of the Carcinoembryonic Antigen Gene Family is Down- Regulated in Colorectal Carcinomas 1. Januar 1994
• Chronic Progressive External Ophthalmoplegia Is Associated with a Novel Mutation in the Mitochondrial tRNA(Asn) Gene 1. Januar 1994
• Clonal Composition of Human Adrenocortical Neoplasms 1. Januar 1994
• Cloning, expression, and chromosomal localization of the 140-kilodalton subunit of replication factor C from mice and humans 1. Januar 1994
• Cutaneos larva migrans in travelers 1. Januar 1994
• Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens 1. Januar 1994
• Exploring the functional domain and the target of the tetanus toxin light chain in neurohypophysial terminals 1. Januar 1994
• Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization 1. Januar 1994
• Expression of the murine cytomegalovirus glycoprotein H by recombinant vaccinia virus 1. Januar 1994
• Fibronectin in human gallbladder bile: cholesterol pronucleating and/or mucin "link" protein? 1. Januar 1994
• Folgen importierter Tropenkrankheiten in Deutschland 1. Januar 1994
• Gene Structure, cDNA Sequence, and mRNA Distribution 1. Januar 1994
• Gnathostomiasis nach Aufenthalt in Thailand. 1. Januar 1994

Alle Episoden von Medizin - Open Access LMU - Teil 11/22 · Mehr Bildung-Podcasts