Inter- and intra-specific gene-density-correlated radial chromosome territory arrangements are conserved in Old World monkeys

Episode von Medizin - Open Access LMU - Teil 14/22 von Ludwig-Maximilians-Universität München · 1. Januar 2005

Recently it has been shown that the gene-density correlated radial distribution of human 18 and 19 homologous chromosome territories (CTs) is conserved in higher primates in spite of chromosomal rearrangements that occurred during evolution. However, these observations were limited to apes and New World monkey species. In order to provide further evidence for the evolutionary conservation of gene-density-correlated CT arrangements, we extended our previous study to Old World monkeys. They comprise the remaining species group to be analyzed in order to obtain a comprehensive overview of the nuclear topology of human 18 and 19 homologous CTs in higher primates. In the present study we investigated four lymphoblastoid cell lines from three species of Old World monkeys by three-dimensional fluorescence in situ hybridization (3D-FISH): two individuals of Japanese macaque ( Macaca fuscata), crab-eating macaque ( Macaca fascicularis), and an interspecies hybrid individual between African green monkey (Cercopithecus aethiops) and Patas monkey ( Erythrocebus patas). Our data demonstrate that gene-poor human 18 homologous CTs are located preferentially close to the nuclear periphery, whereas gene-dense human 19 homologous CTs are oriented towards the nuclear center in all cell lines analyzed. The gene-density-correlated positioning of human 18 and 19 homologous CTs is evolutionarily conserved throughout all major higher primate lineages, despite chromosomal inversions, fusions, fissions or reciprocal translocations that occurred in the course of evolution in these species. This remarkable preservation of a gene-density-correlated chromatin arrangement gives further support for a functionally relevant higher-order chromatin architecture. Copyright (C) 2005 S. Karger AG, Basel.

Weitere Episoden von „Medizin - Open Access LMU - Teil 14/22“

• A role for MCP-1/CCR2 in interstitial lung disease in children 1. Januar 2005
• A simple approach for protein name identification: prospects and limits 1. Januar 2005
• An extrahippocampal projection from the dentate gyrus to the olfactory tubercle 1. Januar 2005
• Association between cognitive performance and cortical glucose metabolism in patients with mild Alzheimer's disease 1. Januar 2005
• Chemokine receptor CCR1: A new target for progressive kidney disease 1. Januar 2005
• Comparing the clinical effectiveness of different new-born hearing screening strategies. A decision analysis 1. Januar 2005
• Concerted upregulation of CLP36 and smooth muscle actin protein expression in human endometrium during decidualization 1. Januar 2005
• Effect of stress doses of hydrocortisone on S-100B vs. interleukin-8 and polymorphonuclear elastase levels in human septic shock 1. Januar 2005
• Expression profiles of hydrophobic surfactant proteins in children with diffuse chronic lung disease 1. Januar 2005
• Fluorescence in situ hybridization to chromosomes as a tool to understand human and primate genome evolution 1. Januar 2005
• Follow-up investigations of tau protein and S-100B levels in cerebrospinal fluid of patients with Creutzfeldt-Jakob disease 1. Januar 2005
• Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis 1. Januar 2005
• Genetic association of CDC2 with cerebrospinal fluid tau in Alzheimer's disease 1. Januar 2005
• Hahnemann's legacy in a new light - A systematic approach to the Organon of Medicine 1. Januar 2005
• 3D Analysis of chromosome architecture: advantages and limitations with SEM 1. Januar 2005

Alle Episoden von Medizin - Open Access LMU - Teil 14/22 · Mehr Bildung-Podcasts