Immunhistochemische, glykohistochemische und ultrastrukturelle Untersuchungen am Ovar der Katze (Felis catus)

Episode von Tierärztliche Fakultät - Digitale Hochschulschriften der LMU - Teil 07/07 von Ludwig-Maximilians-Universität München · 12. Juli 2014

In my thesis 25 feline ovaries (Felis catus) were studied using histological,

glycohistochemical, immunhistochemical and ultrastructural methods.

Additionally to the different follicle stages, the ovarian stroma and the thecal

glands were also evaluated.

For the glycohistochemical investigations, an appropriate panel of lectins was

used, including Concanavalin Agglutinin (Con A), Wheat germ Agglutinin

(WGA), Wheat germ Agglutinin succinylated (WGAs), Sambucus nigra

Agglutinin (SNA) , Pisum sativum Agglutinin (PSA), Ricinus communis

Agglutinin (RCA), Viscum album Agglutinin (VAA), Phaseolus vulgatis

Erythroagglutinin (PHA E), Maackia amurensis Agglutinin I (MAA I), Phaseolus

vulgaris Leukoagglutinin (PHA L), Sophora japonica Agglutinin (SJA) and

Griffonia simplicifolia Agglutinin I (GSA I). The most interesting

glycohistochemical staining was the strong reaction of WGA in the zona pellucida

and the surrounding corona radiata cells. The staining with WGA-FITC

demonstrates N-acetylglucosamine and sialic acids in the zona pellucida and the

surrounding corona radiata.

The immunhistochemical examination using antibodies against cytokeratins,

vimentin, laminin, desmin, synemin, tubulin, SMA, S100, connexin 43, ERα and

progesterone receptors showed the localization of cytoskeletal components within

the different compartments of the feline ovary and the distribution of steroid

hormone receptors. The ovarian surface epithelium contains not only cytokeratins

but also synemin and nuclear estrogen receptors. The interstitial gland cells show

a strong immunohistochemical staining with antibodies against vimentin, S100

and connexin 43. A similar immunohistochemical staining pattern was also

observed in cells of the theca interna of tertiary follicles. The

immunohistochemical staining pattern differs between the luteal cells derived

from granulosa cells (granulosa lutein cells) or from thecal cells (theca lutein

cells). The small theca cells only showed a distinct reaction with tubulin and S100

antibodies, in contrast to the large luteal cells which reacted much more strongly.

In the rete ovarii the expression of cytokeratins, tubulin, progesterone receptors

and ERα could be immunohistochemically demonstrated. Oocytes of the follicles

of different developmental stages only showed a positive reaction with the

synemin antibody. At all developmental stages, the follicle cells showed a strong

immunohistochemical staining with the tubulin antibody. The intensity of

connexin immunostaining increased during follicular development within the

follicular epithelium from primordial to tertiary follicles. This proves the increase

in the number of gap junctions in the follicular epithelium during follicle growth.

In contrast the staining intensity with the vimentin antibody decreased in

granulosa cells from primordial to tertiary follicles. This may indicate the

remodelling of the cytoskeleton of the granulosa cells in growing follicles to

obtain steroidogenic potential.

The formation of nuclear ERα and progesterone receptors varies under the

influence of different hormones, depending on the ovarian cycle. The rete ovarii is

the only structure in which both receptor types are expressed. ERα can be detected

in granulosa cells of some primary follicles, as well as in the ovarian surface

epithelium. Progesterone receptors are localized in the theca interna of tertiary

follicles and in small luteal cells and fibrocytes of the corpus luteum.

In conclusion the feline ovary shows, in comparison to other mammals, an early

differentiation of cytoskeletal and glykan containing elements. The zona pellucida

is already formed in primary follicles and the granulosa cells contain vimentin

filaments and gap junctions, which can already be observed in the stage of

primordial follicle. The distribution of the S100 protein in the ovary of the cat

differs fundamentally from all other species investigated so far. S100 was

predominantly found in steroidogenic cells and may indicate its involvement in

steroidogenesis, in conjunction with the expression of vimentin and connexin.

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