Involvement of Nitric Oxide in Microcirculatory Reactions after Ischemia-Reperfusion of the Rat Urinary Bladder

Episode von Medizin - Open Access LMU - Teil 16/22 von Ludwig-Maximilians-Universität München · 1. Januar 2009

Background: Nitric oxide ( NO) plays a role in inflammation. Our aim was to investigate the role of NO in the microcirculatory changes after ischemia-reperfusion (I/R) of the bladder using intravital videomicroscopy (IVM). Methods: In rats, 60 min of bladder ischemia followed by 30 min of reperfusion was performed in the presence of N(G)-nitro-L-arginine methyl ester (L-NAME), the NO precursor L-arginine, or saline pre-treatments. Venular red blood cell velocity (RBCV), functional capillary density (FCD), vessel diameters, and leukocyte-endothelial cell interactions in postcapillary venules were determined. Concentrations of nitrite/nitrate in the plasma and myeloperoxidase (MPO) levels in the lungs and the bladder were measured. Results: Elevations of the numbers of rolling and adherent leukocytes, and of plasma nitrite/nitrate levels were found, while FCD and RBCV decreased. L-NAME pretreatment ameliorated the enhanced leukocyte-endothelial cell interactions without influencing the microcirculatory perfusion. In contrast, the L - arginine pretreatment further increased plasma nitrite/nitrate levels and preserved the FCD and RBCV, but did not affect leukocyte-endothelial interactions. None of these treatments influenced MPO activities. Conclusion: Our results suggest that NO plays an enhancing role in the I/R-induced neutrophil-endothelial interactions of the bladder. Supplementation of NO ameliorates the microcirculatory perfusion deficit without influencing the postischemic microcirculatory inflammatory reactions. Copyright (c) 2008 S. Karger AG, Basel

Weitere Episoden von „Medizin - Open Access LMU - Teil 16/22“

• An Evolutionary Interpretation of Teleostean Forebrain Anatomy 1. Januar 2009
• Auditory Processing in Children with Specific Language Impairments: Are there Deficits in Frequency Discrimination, Temporal Auditory Processing or General Auditory Processing? 1. Januar 2009
• Beurteilung des Therapieerfolgs – konventionelle versus neue Methoden 1. Januar 2009
• Botulinum Toxin as Preventive Treatment for Migraine: A Randomized Double-Blind Study 1. Januar 2009
• Chromosome Centromeres: Structural and Analytical Investigations with High Resolution Scanning Electron Microscopy in Combination with Focused Ion Beam Milling 1. Januar 2009
• Cisplatin-Based Chemotherapy for Pulmonary Metastasized Germ Cell Tumors of the Testis - Be Aware of Acute Respiratory Distress Syndrome 1. Januar 2009
• Correlation of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinase Expression in Ileal Carcinoids, Lymph Nodes and Liver Metastasis with Prognosis and Survival 1. Januar 2009
• Current Oncological Treatment of Patients with Pancreatic Cancer in Germany: Results from a National Survey on behalf of the Arbeitsgemeinschaft Internistische Onkologie and the Chirurgische Arbeitsgemeinschaft Onkologie of the Germany Cancer Society 1. Januar 2009
• Design and Fabrication of Three-Dimensional Scaffolds for Tissue Engineering of Human Heart Valves 1. Januar 2009
• Destruxin E Decreases Beta-Amyloid Generation by Reducing Colocalization of Beta-Amyloid-Cleaving Enzyme 1 and Beta-Amyloid Protein Precursor 1. Januar 2009
• Development and analytical performance evaluation of an automated chemiluminescent immunoassay for pro-gastrin releasing peptide (ProGRP) 1. Januar 2009
• Diagnostik und Therapie von Lebermetastasen bei kolorektalem Primärtumor 1. Januar 2009
• Die Evidenz der Klima- und Thalassotherapie 1. Januar 2009
• Die Homöopathie im Spannungsfeld zwischen aristotelischer und moderner Wissenschaft 1. Januar 2009
• A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization 1. Januar 2009

Alle Episoden von Medizin - Open Access LMU - Teil 16/22 · Mehr Bildung-Podcasts