Effect of dsRNA on Mesangial Cell Synthesis of Plasminogen Activator Inhibitor Type 1 and Tissue Plasminogen Activator

Episode von Medizin - Open Access LMU - Teil 16/22 von Ludwig-Maximilians-Universität München · 1. Januar 2009

Background/Aims: Viral infections are a major problem worldwide and many of them are complicated by virally induced glomerulonephritides. Progression of kidney disease to renal failure is mainly attributed to the development of renal fibrosis characterized by the accumulation of extracellular matrix components in the mesangial cell compartment and the glomerular basement membrane. Plasminogen activator inhibitor type 1 (PAI-1) and tissue plasminogen activator (t-PA) are major regulators of plasmin generation and play an important role in generation and degradation of glomerular extracellular matrix components. Viral receptors expressed by mesangial cells (MC) are known to be key mediators in immune-mediated glomerulonephritis. We investigated the effect of stimulation of the viral receptors toll-like receptor 3 (TLR3) and retinoic acid-inducible gene I (RIG-I) on the expression of PAI-1 and t-PA. Methods: Expression of PAI-1 and t-PA in immortalized human MC stimulated with polyriboinosinic: polyribocytidylic acid {[}poly(I:C)] RNA and cytokines were analyzed by real-time RT-PCR and ELISA. Results: Incubation of MC with poly(I:C) RNA to activate the viral receptors TLR3 and RIG-I upregulates the expression of PAI-1 and t-PA. Knockdown of viral receptors with specific siRNA abolishes the induction of PAI-1 and t-PA. Conclusion: For the first time a link between the activation of viral receptors on MC and potentially causative agents in the development of glomerulosclerosis and tubulointerstitial fibrosis is shown. The progression of inflammatory processes to glomerulosclerosis can be postulated to be directly enhanced by viral infection. Copyright (C) 2009 S. Karger AG, Basel

Weitere Episoden von „Medizin - Open Access LMU - Teil 16/22“

• An Evolutionary Interpretation of Teleostean Forebrain Anatomy 1. Januar 2009
• Auditory Processing in Children with Specific Language Impairments: Are there Deficits in Frequency Discrimination, Temporal Auditory Processing or General Auditory Processing? 1. Januar 2009
• Beurteilung des Therapieerfolgs – konventionelle versus neue Methoden 1. Januar 2009
• Botulinum Toxin as Preventive Treatment for Migraine: A Randomized Double-Blind Study 1. Januar 2009
• Chromosome Centromeres: Structural and Analytical Investigations with High Resolution Scanning Electron Microscopy in Combination with Focused Ion Beam Milling 1. Januar 2009
• Cisplatin-Based Chemotherapy for Pulmonary Metastasized Germ Cell Tumors of the Testis - Be Aware of Acute Respiratory Distress Syndrome 1. Januar 2009
• Correlation of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinase Expression in Ileal Carcinoids, Lymph Nodes and Liver Metastasis with Prognosis and Survival 1. Januar 2009
• Current Oncological Treatment of Patients with Pancreatic Cancer in Germany: Results from a National Survey on behalf of the Arbeitsgemeinschaft Internistische Onkologie and the Chirurgische Arbeitsgemeinschaft Onkologie of the Germany Cancer Society 1. Januar 2009
• Design and Fabrication of Three-Dimensional Scaffolds for Tissue Engineering of Human Heart Valves 1. Januar 2009
• Destruxin E Decreases Beta-Amyloid Generation by Reducing Colocalization of Beta-Amyloid-Cleaving Enzyme 1 and Beta-Amyloid Protein Precursor 1. Januar 2009
• Development and analytical performance evaluation of an automated chemiluminescent immunoassay for pro-gastrin releasing peptide (ProGRP) 1. Januar 2009
• Diagnostik und Therapie von Lebermetastasen bei kolorektalem Primärtumor 1. Januar 2009
• Die Evidenz der Klima- und Thalassotherapie 1. Januar 2009
• Die Homöopathie im Spannungsfeld zwischen aristotelischer und moderner Wissenschaft 1. Januar 2009
• A Technical Note on Quantum Dots for Multi-Color Fluorescence in situ Hybridization 1. Januar 2009

Alle Episoden von Medizin - Open Access LMU - Teil 16/22 · Mehr Bildung-Podcasts