Cell-Free Synthesis of the Mitochondrial ADP/ATP Carrier Protein of Neurospora crassa

Episode von Medizin - Open Access LMU - Teil 03/22 von Ludwig-Maximilians-Universität München · 1. September 1979

ADP/ATP carrier protein was synthesized in heterologous cell-free systems programmed with Neurospora poly(A)-containing RNA and homologous cell-free systems from Neurospora. The apparent molecular weight of the product obtained in vitro was the same as that of the authentic mitochondrial protein. The primary translation product obtained in reticulocyte lysates starts with formylmethionine when formylated initiator methionyl-tRNA (fMet-tRNAfMet) was present. The product synthesized in vitro was released from the ribosomes into the postribosomal supernatant.

The evidence presented indicates that the ADP/ATP carrier is synthesized as a polypeptide with the same molecular weight as the mature monomeric protein and does not carry an additional sequence.

Weitere Episoden von „Medizin - Open Access LMU - Teil 03/22“

• Automated quantitative analysis of single and double label autoradiographs 1. Januar 1979
• Detection of antibodies to Sendai virus by enzyme-linked immunosorbent assay (ELISA) 1. Januar 1979
• Die blande Struma 1. Januar 1979
• Generation of virus-specific cytotoxic T cells in vitro I. Induction conditions of primary and secondary Sendai virus-specific cytotoxic T cells 1. Januar 1979
• Localized ultraviolet laser microbeam irradiation of early Drosophila embryos: Fate maps based on location and frequency of adult defects 1. Januar 1979
• Transit times through the cycle phases of jejunal crypt cells of the mouse 1. Januar 1979
• Unscheduled DNA synthesis after partial UV irradiation of the cell nucleus 1. Januar 1979
• Zerebrale Manifestationen bei angioneurotischem Ödem 1. Januar 1979
• Zur Diagnose eingeschleppter viszeraler Leishmaniosen (Kala-Azar) 1. Januar 1979

Alle Episoden von Medizin - Open Access LMU - Teil 03/22 · Mehr Bildung-Podcasts