Investigation of the mutagenic potential of naturally occurring oxidized DNA nucleobase derivatives

Episode von Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 05/06 von Ludwig-Maximilians-Universität München · 27. Juni 2013

The integrity of DNA in living organisms is permanently threatened by many endogenous and exogenous factors. Damages can lead to cell death and serious diseases and have to be repaired by the organism. The focus of this thesis is the investigation of the ability of high (Bst Pol I, Klenow fragment exo-) and low fidelity polymerases (Pol kappa, Pol eta) to insert and bypass several oxidatively-derived purine-lesions, such as 8-oxopurines (8-oxodA, 8-oxodG) and formamidopyrimidines (FaPydA, FaPydG), but also guanine-derived imidazolone (dIz) and its further degradation products oxazolone and guanidinoformimine. In order to support the biochemical data and to reveal the replication mechanism of a high fidelity polymerase with FaPy-lesions we performed co-crystallization studies with Bst Pol I. After the discovery that oxidative modifications of the base methylcytosine (mC) occur naturally within the genome and play a key role during cellular development we sought to address the question of their mutagenic potential.

Weitere Episoden von „Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 05/06“

• Transcriptome surveillance in S. cerevisiae by RNA synthesis and degradation coupling and selective termination of non-coding RNAs 9. Oktober 2013
• Regulation der ER-Translokation sekretorischer Proteine durch Sekundärstrukturelemente der Polypeptidkette 8. August 2013
• Protein particle analysis 26. Juli 2013
• Mechanism of cancer evading metronomic chemotherapy and action of Archazolid as an anti-metastatic drug 27. Juni 2013
• Genetic analysis of Drosophila adult muscle type specification 7. Juni 2013

Alle Episoden von Fakultät für Chemie und Pharmazie - Digitale Hochschulschriften der LMU - Teil 05/06 · Mehr Bildung-Podcasts