Modulation of cardiac Ca2+ channels in Xenopus oocytes by protein kinase C
Episode von Chemie und Pharmazie - Open Access LMU - Teil 02/02 von Ludwig-Maximilians-Universität München · 1. Januar 1992
L-Type calcium channel was expressed in Xenopus laevis oocytes injected with RNAs coding for different cardiac Cu2+ channel subunits, or with total heart RNA. The effects of activation of protein kinase C (PKC) by the phorbol ester PMA (4β-phorbol 12-myristate 13-acetate) were studied. Currents through channels composed of the main (1) subunit alone were initially increased and then decreased by PMA. A similar biphasic modulation was observed when the 1 subunit was expressed in combination with 2/δ, β and/or γ subunits, and when the channels were expressed following injection of total rat heart RNA. No effects on the voltage dependence of activation were observed. The effects of PMA were blocked by staurosporine, a protein kinase inhibitor. β subunit moderated the enhancement caused by PMA. We conclude that both enhancement and inhibition of cardiac L-type Ca2+ currents by PKC are mediated via an effect on the 1 subunit, while the β subunit may play a mild modulatory role.
Weitere Episoden von „Chemie und Pharmazie - Open Access LMU - Teil 02/02“
- A Stepwise [4 + 3] Cycloaddition Reaction of the 1,3-Diphenyl-2-azaallyl Anion 1. Januar 1993
- How nucleophilic are silyl enol ethers? Kinetics of the reactions of electron rich CC-double bonded systems with carbenium ions 1. Januar 1993
- Photophysics, Molecular Reorientation in Solution and X-Ray Structure of a New Fluorescent Probe 1,7-Diazaperylene 1. Januar 1993
- Primary structure and functional expression of a cyclic nucleotidegated channel from rabbit aorta 1. Januar 1993
- Tissue-specific expression of calcium channels 1. Januar 1993
- Coordination chemistry of perhalogenated cyclopentadienes and alkynes. 8. Pentakis(dimethylsilyl)cymantrene 1. Januar 1992
- Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes. 1. Januar 1992
- Intrazeolite assembly and pyrolysis of polyacrylonitrile 1. Januar 1992
- Kohlenwasserstoffverbrückte Komplexe, XXV 1. Januar 1992
- Komplexchemie perhalogenierter Cyclopentadiene und Alkine 1. Januar 1992
- Influenza virus hemagglutinin HA-2 N-terminal fusogenic peptides augment gene transfer by transferrin-polylysine-DNA complexes. Toward a synthetic virus-like gene-transfer vehicle. 1. Januar 1992
- Koordinationschemie perhalogenierter Cyclopentadiene und Alkine, X 1. Januar 1992
- Labelling of liposomes with intercalating perylene fluorescent dyes 1. Januar 1992
- Calcium channel beta subunit heterogeneity. functional expression of cloned cDNA from heart, aorta and brain. 1. Januar 1992
- DNA-binding transferrin conjugates as functional gene-delivery agents: synthesis by linkage of polylysine or ethidium homodimer to the transferrin carbohydrate moiety 1. Januar 1991
Alle Episoden von Chemie und Pharmazie - Open Access LMU - Teil 02/02 · Mehr Bildung-Podcasts